It follows an incident on Tuesday (27/10).
Thames Valley Police are appealing for witnesses after a collision on Bellingham Way at the junction with Birmingham Drive at around 5.46pm.
It involved a grey Volvo EX40 and a Kawasaki motorcycle, with a 34-year-old motorcyclist - who remains in hospital - suffering serious leg injuries.
Investigating officer PC Jessica Howells, of the Roads Policing Unit, said: “We are carrying out enquiries to establish exactly what happened and are appealing to anybody who witnessed this collision to come forward.
“The incident occurred during the evening rush hour on a busy route through Aylesbury and we believe there may have been a number of people in the area who saw what happened but left before officers arrived.
“If you witnessed the collision or have any dash-cam footage from the area around the time of the incident, please contact Thames Valley Police.
“Any information, no matter how small it may seem, could assist our investigation.”
Anyone with information is encouraged to call 101 or make a report online, quoting reference number 43260562662.
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